The Imaging facility: Image UP

ImageUP gathers various materiel resources in the field of imaging. In particular ImageUP
aims to facilitate the provision and use of the imaging tools to the entire scientific
community at the site of the University of Poitiers. Thanks to the presence of dedicated
engineers and technicians. ImageUP provides, in addition to equipment availability,
technical expertise and know-how to operate it in the best of its technical capabilities. This
facility is heavily used by our laboratory; it is under the responsibility of Anne Canterau, a
CNRS engineer and Emile Béré, a university engineer who both dedicate almost all of their
working time to this facility with the help of 2 technicians.
This platform includes :

The flow cytometry and cell sorting service regrouping the BD FACSVerse™ flow
cytometer and the BD FACSAria™ III sorter. The main applications are phenotyping
of cells (membrane and intracellular antigens), measurement of apoptosis and/or cell
viability, DNA quantification, the extent of cell proliferation…

LUMINA III XR PerkinElmer Imager. This small animal imaging equipment coupled
to a gas anesthesia system is used for performing in vivo bioluminescence and
fluorescence measurements. This material offers the possibility of a space-time
tracking of biological processes noninvasively, directly within a living organism. The
bioluminescence allows to analyze the regulation of an endogenous gene or to monitor
the growth and migration of tumor cells or an infection. It is used in our laboratory to
monitor for instance angiogenesis or transplanted cells within the cortex.

• The MACROscopy service using the MacroView MVX10 Olympus allows
fluorescence observation of small organisms and whole tissue sections (such as brains).
It combines maximum detection efficiency (resolution) and a wide field imaging.

• Olympus IX2 inverted microscope and Andor revolution confocal microscopy
system constitute the confocal microscopy service regrouping. Samples can be
observed simultaneously or independently according to 3 modes: transmission,
reflection or fluorescence. This set of equipment is well suited for the observation of
thin samples (and up to 100μm) such as cell cultures with an increase of the lateral and
axial resolution.

JEOL JEM-1010 transmission electron microscope (TEM) and JEOL 840A scanning
electron microscope (SEM) constitute the electronic microscopy service. These
microscopes have a higher resolving power than a light microscope and can reveal the
structure of smaller objects. Electron microscopes are used to investigate the
ultrastructure of a wide range of biological and inorganic specimens including
microorganisms, cells, large molecules and biopsy samples. In our laboratory, they are
used to determine the integration of the grafted neurons in term of synapse formation
and myelination.

• The ion conductance microscopy service allows performing images of the surface of
objects (topography) and could be performed on living cells. In addition, since it allows
obtaining position values in z, it’s possible to perform volume calculations.

EasyTom XL duo and Viscom X8050-16 together form the microtomography service.
These systems allow recording a series of digital radiographic projections of a rotating
sample. The data obtained permits the mathematical reconstruction of a section
and/or volume, real maps 2D/3D according to local variations in absorption of X-rays
in the sample. The internal structure of the sample can then be shelled qualitatively
(transparency, virtual cuts, segmentation and virtual extraction) and quantitatively
(dimensional measurements, surface, volumetric). For instance, this service allows
analyzing vascularization of tissues in animals.