HPLC platform

Manager : Virginie Lardeux

Our HPLC platform is composed of two independent HPLC systems with an electrochemical
detector (ESA, Coulochem III and Antec Decade II) equipped with automatic injection
systems. A computer controls the injection and chromatographic analysis of samples. This
system was bought in 2010 and 2011 thanks to CPER credits. This
platform is mainly used by team 2 (M. Solinas), in order to measure neurotransmitters and
their metabolites either in whole brain tissue or following in vivo by microdialysis. These
procedures allow to measure in the same samples three main catecholamines (dopamine,
serotonin and noradrenaline) in several brain structures (dorsal striatum, nucleus
accumbens, cortex…).

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