Microscopy platform

Light mycroscopy :

Manager : Sebastien BROT

-Zeiss Upright AxioImager M2 with apotome (Carl Zeiss):

This is a system for fluorescence and bright field Imaging. Apotome creates optical sections of fluorescent
samples and provides images with high contrast in the best possible resolution. This
system is an alternative to the confocal microscope, thereby allowing us to obtain faster
(and cheaper) results. This system was purchased in 2011 on ANR/FEDER funds.
In 2015, we upgraded this system by the acquisition of a more performing camera (ORCAFlash4.0
V2, Hamamatsu) thanks to a CPER funding. This system is used by all the
members of the unit and allows, for example, for the realizing of mosaic acquisitions with 4
different channels and different Z positions

– Axio Zoom.V16 (Carl Zeiss) is an apochromatic on-axis zoom microscope that delivers
both high resolution and a zoom range of 16x. The objective aperture of Axio Zoom.V16
is big compared to stereomicroscopes and leads to resolution rates of high quality,
particularly at low to medium magnifications. This system was purchased in 2011 on
ANR/FEDER credits and is used by all members of the unit. It allows, for
example, imaging of a whole brain section in a few seconds.
– Mercator station (Explora Nova) is a microscope including a comprehensive software
package for quantification of histological sections. Mercator enables exhaustive
exploration of large histological preparations. After defining the outlines of regions of
interest at low magnification, the user can quantify the cells at high magnification by
using the previously drawn regions. Each cell is then measured to assess morphologic,
colorimetric and densitometric criteria. In addition to real-time localization of the field
of view, Mercator also eliminates the risk of double counting, and can generate highresolution
maps. This system was purchased in 2011 with CPER/FEDER grants (100 k€).
This system is used by all members of the unit. For example, we used this station to
quantify vessel density in a specific brain area, like transplants after grafting in mouse
models (Team 1).

mercator

image-fluo-copie dble-comptage-copie

im-axio-zoom-copie

 

Electron Microscopy :

Manager : Emile BERE

To study the neuro anatomical integration of transplants, transmission electron microscope JEOL 1010 is used to assess synaptic contacts formation between neurons and transplanted neurons  to the host after pre-embedding immuno-labeling of RD1.

JEM 1010 is located on the ImageUP platform with following characteristics:
◦Résolution point by point  :  0.20 nm
◦ Acceleration voltage : 40 to 100 Kv,
◦Magnification :  50x to 600 000x,
◦ emission gun: tungsten Cathode ,
◦Digital Camera :   Quemesa Olympus Soft Imaging Solutions allowing 11 million pixels

Met

marq EM2 marq EM

 

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